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GenScript corporation 4-12% expressplustm page gel m41215c
4 12% Expressplustm Page Gel M41215c, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/page+gels+expressplustm+4~12/12++express+plustm+page+gel+m01210/10__7150_slash_ijms__108187-120-11-15
Average 90 stars, based on 1 article reviews
4-12% expressplustm page gel m41215c - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Remodeling of Mitochondria–Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation
Article Snippet: After heating at 70 °C for 10 min, 30 μg of proteins was loaded onto precast gels (ExpressPlusTM PAGE Gel, Genscript 4–12% or 4–20%, Piscataway, NJ, USA).

Article Title: A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome
Article Snippet: Protein concentration was determined by using a PierceTM BCA Protein Assay kit (Thermo ScientificTM) and 20 μg protein was mixed with SDS-containing reducing sample buffer (Thermo ScientificTM), followed by denaturation at 95 °C for 10 min and loading on 4–20% ExpressPlusTM PAGE gels (GenScript).

Article Title: A deep phenotyping study in mouse and iPSC models to understand the role of oligodendroglia in optic neuropathy in Wolfram syndrome.
Article Snippet: Protein concentration was determined by using a Pierce BCA Protein Assay kit (Thermo Scientific) and 20 μg protein was mixed with SDS-containing reducing sample buffer (Thermo Scientific), followed by denaturation at 95 °C for 10 min and loading on 4–20% ExpressPlus PAGE gels (GenScript).

Article Title: Remodeling of Mitochondria-Endoplasmic Reticulum Contact Sites Accompanies LUHMES Differentiation.
Article Snippet: After heating at 70 ◦C for 10 min, 30 μg of proteins was loaded onto precast gels (ExpressPlusTM PAGE Gel, Genscript 4–12% or 4–20%, Piscataway, NJ, USA).

Electrophoresis:

Article Title: Targeting HIF-1α with Specific DNA Yokes for Effective Anticancer Therapy.
Article Snippet: Hypoxia, a ubiquitous hallmark in cancer, underscores the significance of targeting HIF-1α, the principal transcriptional factor of hypoxic responses, for effective cancer therapy.. Herein, we introduce DNA yokes, a novel class of DNA nanomaterials harboring specific HIF-1α binding sequences (hypoxia response elements, HREs), as nano-pharmaceuticals for cancer treatment.. Comprising a basal tetrahedral DNA nanostructure (TDN) and four HRE-bearing overhanging chains, DNA yokes exhibit exceptional stability and prolonged intracellular retention.

Polyacrylamide Gel Electrophoresis:

Article Title: Targeting HIF-1α with Specific DNA Yokes for Effective Anticancer Therapy.
Article Snippet: Hypoxia, a ubiquitous hallmark in cancer, underscores the significance of targeting HIF-1α, the principal transcriptional factor of hypoxic responses, for effective cancer therapy.. Herein, we introduce DNA yokes, a novel class of DNA nanomaterials harboring specific HIF-1α binding sequences (hypoxia response elements, HREs), as nano-pharmaceuticals for cancer treatment.. Comprising a basal tetrahedral DNA nanostructure (TDN) and four HRE-bearing overhanging chains, DNA yokes exhibit exceptional stability and prolonged intracellular retention.

Article Title: Cardiac-specific deletion of heat shock protein 60 induces mitochondrial stress and disrupts heart development in mice.
Article Snippet: Congenital heart diseases are the most common birth defects around the world.. Emerging evidence suggests that mitochondrial homeostasis is required for normal heart development.. In mitochondria, a series of molecular chaperones including heat shock protein 60 (HSP60) are engaged in assisting the import and folding of mitochondrial proteins.

Article Title: Discovery and Characterization of Active CBP/EP300 Degraders Targeting the HAT Domain.
Article Snippet: LP1 or MM1S cells were harvested in RIPA buffer (50 mM Tris pH 7.4, 2 mM EDTA, 150 mM NaCl, 0.5 % sodium deoxycholate, 1 % Triton X100) containing cOmpleteTM Protease Inhibitor Cocktail (Roche). .. Equivalent amounts of protein were resolved by Western blotting using 4-12 % ExpressPlusTM PAGE gels (GenScript) prior to transfer to PVDF. .. Antibodies against CBP (sc-7300, Santa Cruz), EP300 (D2X6N, Cell Signalling), and vinculin (42H89L44, Invitrogen) were used for protein detection, followed by HRPconjugated secondary antibodies (Southern Biotech) and the enhanced chemiluminescent HRP substrate SuperSignal West Pico PLUS (Thermo Scientific) for signal readout.

Article Title: Structure-Based Design of CBP/EP300 Degraders: When Cooperativity Overcomes Affinity
Article Snippet: Cells were harvested on ice, washed in PBS and lysed in RIPA buffer (50 mM tris pH 7.5, 2 mM EDTA, 150 mM NaCl, 0.5 % sodium deoxycholate, 1 % Triton X100) supplemented with cOmpleteTM protease inhibitor cocktail (Roche). .. Protein concentrations were determined using the PierceTM BCA Protein Assay Kit (Thermo Scientific, 23227) and equal amounts were separated on 4-12 % ExpressPlusTM PAGE gels (GenScript, M41215). .. Proteins were transferred to PVDF membranes, the membranes were blocked with 5 % milk (Merk Millipore, 115363) in PBS-T and proteins were stained with the following antibodies at 4 °C overnight: CBP (Santa Cruz, sc-7300, diluted 1:200), EP300 (Cell Signalling, D2X6N, diluted 1:1000), BRD4 (Bethyl Laboratories, A301-985A50, diluted 1:2000) and vinculin (Invitrogen, 42H89L44, diluted 1:1000).

Membrane:

Article Title: Cardiac-specific deletion of heat shock protein 60 induces mitochondrial stress and disrupts heart development in mice.
Article Snippet: Congenital heart diseases are the most common birth defects around the world.. Emerging evidence suggests that mitochondrial homeostasis is required for normal heart development.. In mitochondria, a series of molecular chaperones including heat shock protein 60 (HSP60) are engaged in assisting the import and folding of mitochondrial proteins.

Western Blot:

Article Title: Discovery and Characterization of Active CBP/EP300 Degraders Targeting the HAT Domain.
Article Snippet: LP1 or MM1S cells were harvested in RIPA buffer (50 mM Tris pH 7.4, 2 mM EDTA, 150 mM NaCl, 0.5 % sodium deoxycholate, 1 % Triton X100) containing cOmpleteTM Protease Inhibitor Cocktail (Roche). .. Equivalent amounts of protein were resolved by Western blotting using 4-12 % ExpressPlusTM PAGE gels (GenScript) prior to transfer to PVDF. .. Antibodies against CBP (sc-7300, Santa Cruz), EP300 (D2X6N, Cell Signalling), and vinculin (42H89L44, Invitrogen) were used for protein detection, followed by HRPconjugated secondary antibodies (Southern Biotech) and the enhanced chemiluminescent HRP substrate SuperSignal West Pico PLUS (Thermo Scientific) for signal readout.

Bicinchoninic Acid Protein Assay:

Article Title: Structure-Based Design of CBP/EP300 Degraders: When Cooperativity Overcomes Affinity
Article Snippet: Cells were harvested on ice, washed in PBS and lysed in RIPA buffer (50 mM tris pH 7.5, 2 mM EDTA, 150 mM NaCl, 0.5 % sodium deoxycholate, 1 % Triton X100) supplemented with cOmpleteTM protease inhibitor cocktail (Roche). .. Protein concentrations were determined using the PierceTM BCA Protein Assay Kit (Thermo Scientific, 23227) and equal amounts were separated on 4-12 % ExpressPlusTM PAGE gels (GenScript, M41215). .. Proteins were transferred to PVDF membranes, the membranes were blocked with 5 % milk (Merk Millipore, 115363) in PBS-T and proteins were stained with the following antibodies at 4 °C overnight: CBP (Santa Cruz, sc-7300, diluted 1:200), EP300 (Cell Signalling, D2X6N, diluted 1:1000), BRD4 (Bethyl Laboratories, A301-985A50, diluted 1:2000) and vinculin (Invitrogen, 42H89L44, diluted 1:1000).



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